Michelle Knowles

  • Biographical Description

  • Publications 1999-2009

    • Michelle K. Knowles, Aurelia Honerkamp-Smith, and Andrew H. Marcus, “Direct measurement of relative and collective diffusion in a dilute binary colloidal suspension.” Journal of Chemical Physics, 2005, 122(23), 234909. doi: 10.1063/1.1926227

      Michelle K. Knowles, Marina G. Guenza, Roderick A. Capaldi, and Andrew H. Marcus, “Cytoskeletal-Assisted Dynamics of the Mitochondrial Reticulum in Living Cells.” Proceedings of the National Academy of Sciences (PNAS), 2002, 99 (23), 14772-14777.doi: 10.1073/pnas.232346999

      Roderick A. Capaldi, R. Aggeler, R. Gilkerson, G. Hanson, M. Knowles, A. Marcus, D.Margineantu, M. Marusich, J. Murray, D. Oglesbee, S.J. Remington, and R. Rossignol, “A replicating module as the unit of mitochondrial structure and functioning.” Biochimica et Biophysica Acta, 2002, 1555, 192-195. doi:10.1016/S0005-2728(02)00277-3

      Tyler J. Grassman, Michelle K. Knowles and Andrew H. Marcus, “Structure and Dynamics of Fluorescently Labeled Complex Fluids by Fourier Imaging Correlation Spectroscopy.” Physical Review E: Statistical, Nonlinear, and Soft Matter Physics, 2000, 62 (6), 8245-8257.doi: 10.1103/PhysRevE.62.8245

      Michelle K. Knowles, Tyler J. Grassman and Andrew H. Marcus, “Measurement of the Dynamic Structure Function of Fluorescently Labeled Complex Fluids by Fourier Imaging Correlation Spectroscopy.” Physical Review Letters, 2000, 85 (13), 2837-2840. doi: 10.1103/PhysRevLett.85.2837

      Book Chapter

      Michelle K. Knowles, Daciana Margineantu, Roderick A. Capaldi and Andrew H. Marcus, “Translational Dynamics of Fluorescently Labeled Species by Fourier Imaging Correlation Spectroscopy.” American Chemical Society, Liquid Dynamics: Into the New Millennium (May2001).

  • Contact Information

    • Office: (303) 871-6698

      Email: michelle.knowles@du.edu

      Office location: S.G. Mudd 101

      Lab location: S.G. Mudd 259

  • Research

    • The goal of our research is to understand the molecular mechanism of membrane proteins. We use biophysical techniques, such as imaging and spectroscopy, to temporally map proteins on the plasma membrane of living cells and model membrane systems. We are particularly interested in how membrane shape can control protein sorting and function. We focus on proteins that facilitate membrane fusion (SNARE proteins), recognize apoptotic cells for removal (Complement proteins), and proteins that regulate the flow of chemicals across the plasma membrane (P-glycoprotein).

  • Publications 2010-current

    • Peer-reviewed Publications

      Kathryn P. Wall, Rebecca Dillon, Michelle K. Knowles. "Fluorescence Quantum Yield Measurements of Fluorescent Proteins: A Laboratory Experiment for a Biochemistry or Molecular Biophysics Laboratory Course." Submitted to Biochemistry and Molecular Biology Education June 2014.

      Joshua C Black, Philip P. Cheney, Travis Campbell, Michelle K. Knowles. "Membrane curvature based lipid sorting using a nanoparticle patterned substrate." Soft Matter, 2014, 10(12), 2016-23. doi: 10.1039/c3sm52522h

      Philip Cheney, Mick Stachler, Michelle K. Knowles. “Single Molecule Tracking of P-glycoprotein in Live Cells Reveals Dynamic Heterogeneity.” IEEE Engineering in Biology and Medicine, 2012, 3159-62. doi: 10.1109/EMBC.2012.6346635

      Min S. Wang, Joshua C. Black, Michelle K. Knowles, and Scott M. Reed.“C-reactive protein (CRP) aptamer binds to monomeric but not pentameric form of CRP.”Analytical and Bioanalytical Chemistry, 2011, 401(4), 1309-18.  doi: 10.1007/s00216-011-5174-1.

      Michelle K. Knowles, Sebastian Barg, L. Wan, M. Midorikawa, X. Chen, and Wolfhard Almers, “Single secretory granules of live cells recruit syntaxin-1 and synaptosomal associated protein25 (SNAP-25) in large copy numbers.”  Proceedings of the National Academy of Sciences (PNAS), 2010, 107 (48), 20810-20815.  doi: 10.1073/pnas.1014840107

      Sebastian Barg, Michelle K. Knowles, Lei Wan, Xi Chen, and Wolfhard Almers. “Syntaxin clusters assemble reversibly at sites of secretory granules in live cells.” Proceedings of the National Academy of Sciences (PNAS), 2010, 107 (48), 20804-20809. doi: 10.1073/pnas.1014823107

      William Birdsong, Leonardo Fierro, Frank G Williams, Valeria Spelta, Ligia A Naves, Michelle K Knowles, Josephine Marsh-Haffner, John P Adelman, Wolfhard Almers, Robert P Elde, Edwin W McCleskey, “Sensing muscle ischemia: coincident detection of acid and ATP via interplay of two ion channels.” Neuron, 2010, 68, 739–749.doi10.1016/j.neuron.2010.09.029

       

       

This portfolio last updated: Jul 9, 2014 4:20:18 PM