I am an associate professor in the Department of Chemistry and Biochemistry and my lab focuses on developing quantitative imaging assays and novel biosensors to understand lipid-protein interactions. We commonly use total internal reflection fluorescence (TIRF) microscopy, confocal microscopy, FRAP and FRET techniques.
I am also involved the interdisciplinary molecular biophysics program that includes members of the Physics, Chemistry and Biochemistry, and Biology Department. More information about Biophysical sciences at the University of Denver can be found here:
Michelle K. Knowles, Aurelia Honerkamp-Smith, and Andrew H. Marcus, “Direct measurement of relative and collective diffusion in a dilute binary colloidal suspension.” Journal of Chemical Physics, 2005, 122(23), 234909. doi: 10.1063/1.1926227
Michelle K. Knowles, Marina G. Guenza, Roderick A. Capaldi, and Andrew H. Marcus, “Cytoskeletal-Assisted Dynamics of the Mitochondrial Reticulum in Living Cells.” Proceedings of the National Academy of Sciences (PNAS), 2002, 99 (23), 14772-14777.doi: 10.1073/pnas.232346999
Roderick A. Capaldi, R. Aggeler, R. Gilkerson, G. Hanson, M. Knowles, A. Marcus, D.Margineantu, M. Marusich, J. Murray, D. Oglesbee, S.J. Remington, and R. Rossignol, “A replicating module as the unit of mitochondrial structure and functioning.” Biochimica et Biophysica Acta, 2002, 1555, 192-195. doi:10.1016/S0005-2728(02)00277-3
Tyler J. Grassman, Michelle K. Knowles and Andrew H. Marcus, “Structure and Dynamics of Fluorescently Labeled Complex Fluids by Fourier Imaging Correlation Spectroscopy.” Physical Review E: Statistical, Nonlinear, and Soft Matter Physics, 2000, 62 (6), 8245-8257.doi: 10.1103/PhysRevE.62.8245
Michelle K. Knowles, Tyler J. Grassman and Andrew H. Marcus, “Measurement of the Dynamic Structure Function of Fluorescently Labeled Complex Fluids by Fourier Imaging Correlation Spectroscopy.” Physical Review Letters, 2000, 85 (13), 2837-2840. doi: 10.1103/PhysRevLett.85.2837
Michelle K. Knowles, Daciana Margineantu, Roderick A. Capaldi and Andrew H. Marcus, “Translational Dynamics of Fluorescently Labeled Species by Fourier Imaging Correlation Spectroscopy.” American Chemical Society, Liquid Dynamics: Into the New Millennium (May2001).
Our lab website: http://michelleknowles.wix.com/knowleslab
Office: (303) 871-6698
Office location: S.G. Mudd 101
Lab location: S.G. Mudd 259
The goal of our research is to understand the molecular mechanism of membrane proteins. We use biophysical techniques, such as imaging and spectroscopy, to temporally map proteins on the plasma membrane of living cells and model membrane systems. We are particularly interested in how membrane shape can control protein sorting and function. We focus on proteins that facilitate membrane fusion (SNARE proteins), recognize apoptotic cells for removal (Complement proteins), and proteins that regulate the flow of chemicals across the plasma membrane (P-glycoprotein).
Kathryn P. Wall, Rebecca Dillon, Michelle K. Knowles. "Fluorescence Quantum Yield Measurements of Fluorescent Proteins: A Laboratory Experiment for a Biochemistry or Molecular Biophysics Laboratory Course." Submitted to Biochemistry and Molecular Biology Education June 2014.
Joshua C Black, Philip P. Cheney, Travis Campbell, Michelle K. Knowles. "Membrane curvature based lipid sorting using a nanoparticle patterned substrate." Soft Matter, 2014, 10(12), 2016-23. doi: 10.1039/c3sm52522h
Philip Cheney, Mick Stachler, Michelle K. Knowles. “Single Molecule Tracking of P-glycoprotein in Live Cells Reveals Dynamic Heterogeneity.” IEEE Engineering in Biology and Medicine, 2012, 3159-62. doi: 10.1109/EMBC.2012.6346635
Min S. Wang, Joshua C. Black, Michelle K. Knowles, and Scott M. Reed.“C-reactive protein (CRP) aptamer binds to monomeric but not pentameric form of CRP.”Analytical and Bioanalytical Chemistry, 2011, 401(4), 1309-18. doi: 10.1007/s00216-011-5174-1.
Michelle K. Knowles, Sebastian Barg, L. Wan, M. Midorikawa, X. Chen, and Wolfhard Almers, “Single secretory granules of live cells recruit syntaxin-1 and synaptosomal associated protein25 (SNAP-25) in large copy numbers.” Proceedings of the National Academy of Sciences (PNAS), 2010, 107 (48), 20810-20815. doi: 10.1073/pnas.1014840107
Sebastian Barg, Michelle K. Knowles, Lei Wan, Xi Chen, and Wolfhard Almers. “Syntaxin clusters assemble reversibly at sites of secretory granules in live cells.” Proceedings of the National Academy of Sciences (PNAS), 2010, 107 (48), 20804-20809. doi: 10.1073/pnas.1014823107
William Birdsong, Leonardo Fierro, Frank G Williams, Valeria Spelta, Ligia A Naves, Michelle K Knowles, Josephine Marsh-Haffner, John P Adelman, Wolfhard Almers, Robert P Elde, Edwin W McCleskey, “Sensing muscle ischemia: coincident detection of acid and ATP via interplay of two ion channels.” Neuron, 2010, 68, 739–749.doi10.1016/j.neuron.2010.09.029